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建立了枳實中柚皮苷、橙皮苷、新橙皮苷、蘆丁、槲皮素含量的測定方法，同時測定了枳實提取物的抗氧化作用。方法采用hplc法，色譜柱：phenomenex gemini c18柱(150 mm×4.6 mm，5 μm)，柱溫37℃，流動相為乙腈-磷酸溶液，檢測波長280，365 nm，流速1.0 ml/min 。采用dpph·測定枳實提取物的自由基清除能力。結(jié)果進樣量范圍：柚皮苷為1.143～5.717 μg，橙皮苷為0.092～0.459 μg，新橙皮苷為1.179～5.895 μg，蘆丁為0.024～0.118 μg，槲皮素為0.079～0.395 μg。柚皮苷、橙皮苷、新橙皮苷的平均回收率分別為99.604%，100.150%，100.249%。rsd分別為0.515%，4.390%，0.774%。枳實提取物對dpph·清除作用是柚皮苷的17.6倍。結(jié)論hplc測定法簡便、準(zhǔn)確，可用于測定枳實類黃酮含量，同時枳實提取物具有較強的自由基清除作用。

【關(guān)鍵詞】 枳實； 類黃酮； 高效液相色譜法； 抗氧化作用  上禾生物

determination of flavoniods in citrus aurantium l. extract by hplc and its antioxidant activity

jiao shi�瞨ong， huang cheng�瞴u

(school of bioengineering of xihua university，chengdu 610039, china.  school of huaxi public health,sichuan university,chengdu 610041,china)

abstract：objectiveto establish an hplc method for determination of naringin, hesperidin, neohesperidin, rutin and quercetin in citrus aurantium l., and to study antioxidant activity .methodsthe samples were extracted with ethanol. the chromatographic conditions were as follows: phenomenex gemini c18 column (150 mm×4.6 mm,5μm), column temperature at 37℃, mobile phase of acetonitrile-phosphate solution, detection wavelength at 280nm or 365nm, flow rate 1.0ml/min. in addition, dpph radical-scavenging was employed to measure antioxidant activities. resultsthe calibration curves were linear in the range of 1.143～5.717μg for naringin, 0.092～0.459μg for hesperidin, 1.179～5.895μg for neohesperidin, 0.024～0.118μg for rutin and 0.079～0.395μg for quercetin. the average recoveries of naringin, hesperidin, neohesperidin were 99.604%, 100.150% and 100.249% respectively. rsd were 0.515%, 4.390% and  0.774% respectively. the dpph radical scavenging activity of c. aurantium l. flavoniods was 17.6 times of naringin. conclusionthe method is simple, accurate for detecting flavoniods in c. aurantium l., and the c.aurantium l .extract has antioxidant activities.

key words：citrus aurantium l. ；  flavoniods ;   high performance liquid chromatography;   antioxidant activity

枳實為蕓香科植物酸橙citrus aurantium l.及其栽培變種或甜橙citrus sinensis osbeck的干燥幼果，具有破氣除痞、化痰消積的功效，是中醫(yī)臨床常用藥物［1］。

關(guān)于測定枳實類黃酮的hplc法大部分報道是測定柚皮苷、橙皮苷［2，3］，而對于新橙皮苷、蘆丁、槲皮素的測定，少有報道，同時對于枳實類黃酮的抗氧化作用未見報道。本研究采用hplc測定枳實中柚皮苷、橙皮苷、新橙皮苷、蘆丁、槲皮素的含量，同時測定了枳實類黃酮對dpph自由基的清除作用，為枳實藥用的深入研究提供基礎(chǔ)。

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