HPLC法測定厚樸中的厚樸酚(Magnolol)和和厚樸酚(Honokiol)含量--上禾生物 | HPLC法測定厚樸中的厚樸酚(Magnolol)和和厚樸酚(Honokiol)含量--上禾生物 |
| 發(fā)布時間:2023-10-08 信息來源:admin 發(fā)布人:admin 點擊次數(shù):295 |
HPLC法測定厚樸中的厚樸酚(Magnolol)和和厚樸酚(Honokiol)含量--上禾生物 1)試劑 a. 重蒸水 b. 甲醇（色譜純） 2)儀器 高效液相色譜儀（Shimadzu） 分析天平（1/10000） 超聲波清洗儀 0.45mm微孔過濾器 50ml容量瓶 帶塞小玻璃瓶 3)色譜條件 色譜柱 Kromasil C18 5mm 4.6×200mm 波長 294nm 流動相 A: 水 (20%) B: 甲醇(80%) 進樣量 10ml 流速 1.0ml/min
4)樣品的制備 （1）待測樣品：精確稱取30mg提取物于50ml容量瓶中，用甲醇溶解，超聲定容，0.45mm微孔濾膜過濾即為供試液。 （2）標準樣品: 精確稱取厚樸酚、和厚樸酚標準品用甲醇溶解,使其濃度為0.2mg/ml, 0.45mm微孔濾膜過濾。 5)計算 根據(jù)各峰峰面積利用外標法計算各種成份的含量。
Determination of Magnolol and Honokiol by HPLC
1) Reagents a. Nanopure water b. Methanol (HPLC) 2) Apparatus HPLC (Shimadzu) Analytical balance (1/10000) Ultrasonic cleaner 0.45μm PTFE filter 50ml volumetric flask Screw cap vials
3) HPLC condition Column: Kromasil C18 5mm 4.6×200mm Detector: UV 294nm Mobile Phase: A= water B= MeOH A:B=20:80(Isocratic) Flow Rate: 1.0ml/min Injection: 10μl
4) Procedure Samples Preparation Accurately weigh 30mg extraction into 50ml volumetric flask. Add MeOH to volume and sonicate to mix, filter through a 0.45μm PTFE filter into a screw cap vial and proceed with the analysis. Standard preparation /calibration Prepare a standard solution (approximately 0.2mg/ml in MeOH) using a standard of Magnolol，Sonicate for about 10 minutes and transfer to screw-cap vials and can be stored in the refrigeration.
5) Calculation Determine the peak areas of the Maglolol on the sample chromatogram and compare to the corresponding peaks in the chromatogram for the standard solution. Calculate the weight percent.
|
